DND-CAT is supported through Northwestern University, The Dow Chemical Company and DuPont de Nemours, Inc. Additional support has been provided by the State of Illinois through the Department of Commerce and the Board of Education (HECA), the US Department of Energy Office of Energy Research, and the US National Science Foundation (NSF) Division of Materials Research. We gratefully acknowledge the receipt of ARRA funding from NSF through the MRI-R2 program for the detector system for station 5IDD.

Link: https://www.dnd.aps.anl.gov/

BioCARS is a national user facility for frontier synchrotron-based dynamics studies in structural biology at the Advanced Photon Source. BioCARS provides state-of-the-art X-ray facility, scientific and technical expertise and support for users to enable their studies of dynamic properties of biological macromolecules by time-resolved diffraction and solution scattering (SAXS/WAXS). BioCARS 14-ID beamline provides high polychromatic flux of ~5.8 × 10^9 photons per 250ps pulse for conducting pump-probe time-resolved experiments. The overall goal is to understand basic biological processes in structural and dynamics terms, on time scales from 250 picoseconds to seconds.

Link: https://biocars.uchicago.edu/

IMCA-CAT is a structural biology research facility for pharmaceutical drug discovery programs. It was established in 1992 to design, build, and operate a research facility at the Advanced Photon Source, a high energy synchrotron X-ray source at Argonne National Laboratory. Today, IMCA-CAT operates a state-of-the-art insertion device beamline for confidential and proprietary macromolecular crystallography experiments. To meet the demands of the pharmaceutical industry, IMCA-CAT is optimized for high-throughput data acquisition, is committed to generating the highest quality X-ray diffraction data, provides reliable access for proprietary research, ensures maximum efficiency, and invests in the facility to remain at the technical forefront for pharmaceutical research in structural biology. 

Link: https://www.imca-cat.org/IMCA-CAT#&panel1-1

The Biophysics Collaborative Access Team’s (BioCAT) mission is to develop and operate a state-of-the-art X-ray facility for the study of the structure and dynamics of biological systems under non-crystalline conditions similar to their functional states in living tissues. BioCAT uses solution small angle X-ray scattering (SAXS), muscle and fiber diffraction, and scanning diffraction to study samples in these conditions.

BioCAT is organized as a National Institutes of Health(NIH) Mature Synchrotron Resource (MSR) and is funded by the National Institute of General Biomedical Sciences (NIGMS). Its primary research tool is a very high brightness X-ray beam-line at the Advanced Photon Source (APS) at Argonne National Laboratory (ANL). BioCAT is a member of Illinois Institute of Technology's (IIT) Center for Synchrotron Radiation Research and Instrumentation (CSSRI) and the Pritzker Institute of Biomedical Science & Engineering.

Link: https://www.bio.aps.anl.gov/

The Structural Biology Center (SBC) at Argonne National Laboratory’s Advanced Photon Source (APS) is a structural biology research unit with particular strength in macromolecular crystallography of proteins, nucleic acids, and biological complexes. SBC also supports sample preparation for a range of structural and molecular biology studies and leverages high-brightness APS X-ray beamlines with high-throughput workflows for data collection, processing, and structure determination. SBC also uses cryo-electron microscopy (cryo-EM), broadening the range of biological systems that can be investigated.

SBC conducts research relevant to energy, health, and the environment, enabling studies of protein–ligand interactions, enzyme mechanisms, and challenges in pathogen and cancer biology. Capabilities include anomalous phasing, automated data collection, cryo- and ambient-temperature crystallography, and expanding options for serial and time-resolved measurements. SBC research is conducted in collaboration with scientists from universities, national laboratories, and research institutes.

https://sbc.aps.anl.gov/

eBERlight is a U.S. Department of Energy’s Office of Science, Biological and Environmental Research (BER) funded program at the APS. The program provides access to a suite of X-ray-based tools and offers comprehensive user support for research in the biological, geological, geochemical, and environmental sciences. The team collaborates closely with users to prepare experiments and works in partnership with other XSD groups and LS-CAT to support a variety of advanced measurement techniques, including: Macromolecular Crystallography (MX), X-ray Microscopy, including X-ray fluorescence microscopy (XFM) and ptychography, X-ray Full-Field Imaging, such as Computed Tomography (CT), X-ray Scattering, including Small- and Wide-Angle X-ray Scattering (SAXS/WAXS), and X-ray Absorption Spectroscopy (XAS).

https://eberlight.aps.anl.gov/

The Life Sciences Collaborative Access Team (LS-CAT) provides macromolecular crystallography resources for those seeking to determine protein structures. LS-CAT provides access to state-of-the-art X-ray diffraction facilities at Argonne National Laboratory's Advanced Photon Source, where extremely intense beams of X-rays are focused onto tiny protein crystals, and the diffracted X-rays are recorded on detectors, producing images needed to calculate where the atoms in the protein crystal are.

LS-CAT was formalized in 2002 with seed money from the State of Michigan through the Michigan Core Technology Alliance. Northwestern University was selected as the managing partner. 

The LS-CAT facilities include four experimental stations using 2 insertion devices. The main beamline (21-ID-D) uses a shortened "Undulator" A and a Kohzu monochromator. The other three experimental stations share a single 3.0-cm undulator. The first two of these stations use diamond Laue monochromators supplied by JJ X-Ray along with beryllium lenses to focus the X-rays onto the sample. This allows SAD experiments to be done at the selenium edge (12.668 KeV).

The Southeast Regional Collaborative Access Team (SER‐CAT) is an organization consisting of 19 Member and 4 Associate User institutions, most of which are located in the Southeastern region of the USA. SER‐CAT is operated by the University of Georgia.

SER‐CAT's beamlines are located at Sector 22, Advanced Photon Source (APS), Argonne National Laboratory (ANL). As a part of the APS Upgrade, they are presently being converted to two state‐of‐the‐art Insertion Device beamlines (22‐ID‐D, variable wavelength; and the new 22‐ID‐E, fixed wavelength). Future emphasis will be placed on de novo SAD/MAD structure determination, high‐resolution structural analyses, micro crystals, large unit cells, drug design, soft X‐ray data collection, full hands‐free automated data collection, and serial synchrotron crystallography.

Link: https://www3.ser.aps.anl.gov/

GM/CA@APS was founded by the National Institute of General Medical Sciences and the National Cancer Institute to build and operate a national user facility for structural biology at the Advanced Photon Source (APS), Argonne National Laboratory. GM/CA’s mission is to support the determination of atomic-resolution structures of biological macromolecules, including proteins and nucleic acids, to address challenging biomedical problems.

The facility provides state-of-the-art X-ray crystallography capabilities, including intense, tunable, micro-focused X-ray beams and highly automated experimental workflows. Distinguishing features of GM/CA include high-speed serial data collection and the use of high-energy X-rays (up to 35 keV), enabling efficient data collection from small, and radiation-sensitive crystals. Beamtime is provided to the biomedical research community through the APS General User Program on a peer-reviewed basis.

Link: https://www.gmca.aps.anl.gov/

The Northeastern Collaborative Access Team (NE-CAT) is a Cornell University-managed facility at the Advanced Photon Source (APS) at Argonne National Laboratory that operates synchrotron X-ray beamlines to address technically challenging problems in structural biology. The facility is funded by its member institutions and the National Institute of General Medical Sciences (NIGMS) and provides more than 75% of our available beam time to the national research community, serving as an important resource for both institutional members and national users. 

Link: https://necat.chem.cornell.edu/

LRL-CAT (Lilly Research Laboratories Collaborative Access Team) is Eli Lilly’s cutting-edge macromolecular crystallography beamline at the Advanced Photon Source. Built for structure-based drug discovery, LRL-CAT delivers high-throughput, high-resolution X-ray diffraction data that speeds the path from crystals to cures. Featuring advanced instrumentation and fully integrated automation, the beamline supports Lilly, General Users, and partner organizations in advancing structural biology and accelerating discovery pipelines. LRL-CAT’s mission is to provide reliable, world-class structural insights that help transform innovative science into life-changing medicines.

Link: https://lrlcat.lilly.com/

The Advanced Protein Characterization Facility (APCF) provides state-of-the-art laboratories for protein production, characterization, and crystallization, closely linked to Argonne’s Advanced Photon Source (APS) to help deliver high-quality crystals to APS X-ray beamlines. Funded by the State of Illinois, APCF attracts researchers nationwide by offering advanced technologies for studying proteins, nucleic acids, complexes, and molecular interactions.

The APCF offers specialized, adaptable laboratory space and automated, computation-ready workflows that strengthen Argonne’s structural biology capabilities and enable research in protein science, systems biology, and synthetic biology.

The protein production laboratories are in APS Sector 84 and are available to users. APS users may apply to use the Sector 84 labs on a cost-recovery basis through the MCSG community resource, following Argonne-approved costing procedures.

Link: https://www.anl.gov/apcf