“We know there has been a lot of drought on the prairies, and people’s livelihoods are on the line,” said Ariana Forand, a master’s degree student in the College of Agriculture and Bioresources at the University of Saskatchewan (USask). “It would be amazing to find modifications that would allow plants to resist multiple stresses.” Forand led a project that explored how calcium and boron play a beneficial role in strengthening plant cell walls, helping reduce the dehydration that comes with freezing and drought and increasing resistance to pathogens. As it turned out, the perfect plant to test the theory on was onions. The team analyzed onion samples and collected data at the U.S. Department of Energy’s Advanced Photon Source (APS), thanks to the facility’s partnership with the Canadian Light Source (CLS) at the University of Saskatchewan.
“This project really builds on the work of a previous USask master’s student, Jun Liu, who did freezing stress work,” said Forand, “and we know that in both drought and cold, plants lose water in similar ways.” Onions are good plants to use “because you can easily peel away a single layer of cells and see the changes in the cell wall,” a plant structure key to protecting against stresses of various kinds.
A unique feature of this research, the results of which were published in the journal Plants, was that it looked at multiple stresses at once – dehydration in Welsh onions and cooking onions, and pathogen resistance in Arabidopsis, a small flowering weed native to Africa.
After adding calcium mixed with water to the greenhouse-grown onions, Forand used synchrotron x-ray microscopy at the X-ray Science Division Spectroscopy Group’s 20-ID x-ray beamline at the APS, an Office of Science user facility at Argonne National Laboratory. The two-dimensional images resulting from the study at the APS were used to plot the calcium map and to increase image quality by eliminating saturated pixels. False color maps were produced using a rainbow color scheme in which the blue-to-red color gradient shows low-to-high relative calcium concentrations (Fig. 1).
The results confirmed not only that the plants had taken up the calcium but that it localized in the cell wall.
Further testing with dry conditions showed a reduction in water loss in the treated plants. Similarly, boron is known to bind with pectin in the cell walls of Arabidopsis, strengthening its resistance to introduced disease.
“We were looking at ways to structurally reinforce cell walls,” said Forand. Confirming that calcium and boron reduce the impact of moisture loss and disease opens the door to looking for a similar effect in other plants.
Karen Tanino, USask professor of plant sciences and Forand’s supervisor, said that in any given year, “one stress could be more prevalent than another–you just can’t really predict what it will be. This research presents a chance to protect plants against stress variations from year to year.”
Both Forand and Tanino believe expanding on their research presents opportunities to strengthen resistance to moisture loss and disease in both field crops and in the horticulture industry. Tanino, the first USask plant science researcher to make use of synchrotron techniques at the CLS in a project that examined freezing resistance in, coincidentally, onions, said that while shoring up plant cell walls against various stresses is not a silver bullet, “it is a first line of defense.”
See: Ariana D. Forand1, Y. Zou Finfrock2, 3, Miranda Lavier3 , Jarvis Stobbs3, Li Qin1 , Sheng Wang1, Chithra Karunakaran3, Yangdou Wei1, Supratim Ghosh1, and Karen K. Tanino1*, “With a Little Help from My Cell Wall: Structural Modifications in Pectin May Play a Role to Overcome Both Dehydration Stress and Fungal Pathogens” Plants 11, 385 (2022). DOI: 10.3390/plants11030385
Author affiliations: 1University of Saskatchewan, 2Argonne National Laboratory, 3Canadian Light Source
Correspondence: * firstname.lastname@example.org
Part of the research described in this paper was performed at the Canadian Light Source, a national research facility of the University of Saskatchewan, which is supported by the Canada Foundation for Innovation (CFI), the Natural Sciences and Engineering Research Council (NSERC), the National Research Council (NRC), the Canadian Institutes of Health Research (CIHR), the Government of Saskatchewan, and the University of Saskatchewan. This research used resources of the Advanced Photon Source, an Office of Science User Facility operated for the U.S. Department of Energy (DOE) Office of Science by Argonne National Laboratory and was supported by the U.S. DOE under Contract No. DE-AC02-06CH11357, and the Canadian Light Source and its funding partners.
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