X-ray fluorescence images of hydrated Pseudomonas fluorescens bacteria treated with chromium (+6) solution; elemental maps of potassium and chromium are shown. Images taken with the 2-ID-D x-ray microprobe at the APS. (Photos courtesy of the Environmental Research Div and the Advanced Photon Source of Argonne.)

Workshop on Biological Applications of X-Ray Microbeams

Argonne National Laboratory

Hosted by XFD and BIO Divisions

Bldg. 362, Rm. F108

May 14-15, 2001







The recent information and technical explosion in biological sciences including massive DNA sequencing of genomes and determination of structures of large numbers of biomolecules has also ushered in an era of bio-visualization and nano-technology. New approaches to characterizing and manipulating intracellular events in all cell types from bacteria to humans are being developed and expanded to afford new opportunities for intracellular and/or nano-scale exploration. 

Hard X-ray microfocusing techniques are emerging as a new and powerful technique for studying biological systems. The recent development of brilliant third generation synchrotron sources, such as the Advanced Photon Source, provide enough signal to allow efficient study of biomaterials including cells and micro-organisms at sub-optical resolution. Using x-ray fluorescent and spectrometric approaches, the elemental and chemical state of trace metals, as well as of major constituents, can be studied quantitatively at high sensitivity and with minimum specimen preparation. The purpose of this workshop is to discuss the current capabilities of the x-ray microbeam and to identify novel applications. 

The one and a half day workshop is sponsored by the XFD and BIO Division of Argonne National Laboratory. Brief overview of key microscopy techniques and current applications of x-ray microbeam will be presented, followed by future applications. Ample time will be allowed for open discussion, and attendee's feedback will be greatly encouraged. 


Gayle Woloschak (BIO), 630-252-3312, woloschak@anl.gov
Barry Lai (APS), 630-252-6405, blai@aps.anl.gov
Jorg Maser (APS), 630-252-1081, maser@aps.anl.gov
Barbara Meurer (APS), 630-252-9742, bmeurer@aps.anl.gov