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Beamline 21-ID-D: Insertion Device Beamline

Life Sciences


Sector 21 uses canted undulators to provide two independent sources for four ID end stations: 21-ID-D, 21-ID-E, 21-ID-F, and 21-ID-G. All four stations can be used simultaneously and independently. The 21-ID-D station supports macromolecular crystallography, SAD and MAD phasing over a wide energy range from 6.5 keV to 20 keV. All LS-CAT experimental stations are equipped with MD-2 diffractometers with state-of-the-art features such as on-axis sample viewing with precision zoom, automatic sample alignment, omega/kappa/phi motions, and a tuned piezoelectric fast shutter.


Supported Techniques

  • Macromolecular crystallography
  • Microfluorescence (hard x-ray)
  • Nano-fluorescence imaging
  • Nano-tomography

Beamline Controls and Data Acquisition

MD-2 diffractometer software from EMBL (with LS-CAT developed extensions) for sample mounting, centering, beamline diagnostics, and data colection. VME EPICS/MEDM low-level interface, PMAC Turbo motion control, and Linus workstations for data collection and processing.


  • Dectris Eiger 9M

Additional Equipment

  • CATS Sample Changer: SPINE or ACTOR pucks
  • MD2 Micro Diffractometer
  • Xradia Bionanoprobe

Special Capabilities

  • In addition to protein crystallography 21-ID-D supports the Bionanoprobe, an instrument designed for 2 and 3 dimensional fluorescence mapping of biological samples.

Local Contacts

Phone 630.252.

Beamline Specs


U33S Undulator

Monochromator Type


Energy Range

6.5-20 keV

Resolution (ΔE/E)

2 x 10 -4

Flux (photons/sec)

5 x 1012 @12 keV

Beam Size (HxV)


10µm x 10µm

For additional information see:

Current Status:

Operational/Accepting General Users

Access Mode:

On-site Remote Mail-in